Abstract
We have used the freeze fracture and cytoplasmic maceration technique to study microsporogenesis inCatananche caerulea L. and here we present preliminary observations of the male germ unit (MGU)in situ. The technique has the advantage of enabling the complex structure of the MGU to be seen in a single fracture plane whereas previously this was only possible by the reconstruction of transmission electron micrographs of serial thin sections. The arrangement of the two generative cells and the vegetative nucleus closely resembles that described from other species includingPlumbago zeylanica.
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