Abstract

Molecular marker-assisted selection can significantly improved resistance breeding efficiency. The soybean frogeye leaf spot (FLS) is caused by the fungus Cercospora sojina Hara. In order to find the resistant markers linked to C. sojina race 7 in soybean, we carried out the resistant genetic ananlysis and the resistant locus mapping. The F 2 population derived from the cross of 'Gang 95144-1' and 'Gongjiao 9723-6' was employed to analyze the resistance genetics. it was sure that the resistance conferring to FLS was controlled by one major dominant gene.. Bulk Segregant Analysis (BSA) and Simple Sequence Repeat (SSR) method were used to map the resistant locus. Among the 600 selected SSR primers, there were four primer pairs exhibiting the polymorphisms between DNA bulk of resistant and susceptible as well as both parents. Segregation distribution of the SSR markers, satt 414 and satt384, were conformed significantly to be as 1:2:1.of Mendel Segregation. The marker, satt411 and satt384 were assigned to link with the resistant locus based on estimation of MAPMAKER/EXP 3.0 software. The linkage order and distance would be Satt384 -5.7cM - Satt411 -7.9cM - Hrcs7 . The resistant locus was integrated to soybean linkage group E based on the Cregen ' s genetic linkage map of soybean.Molecular marker-assisted selection can significantly improved resistance breeding efficiency. The soybean frogeye leaf spot (FLS) is caused by the fungus Cercospora sojina Hara. In order to find the resistant markers linked to C. sojina race 7 in soybean, we carried out the resistant genetic ananlysis and the resistant locus mapping. The F 2 population derived from the cross of 'Gang 95144-1' and 'Gongjiao 9723-6' was employed to analyze the resistance genetics. it was sure that the resistance conferring to FLS was controlled by one major dominant locus named Hrcs7 .. Bulk Segregant Analysis (BSA) and Simple Sequence Repeat (SSR) method were used to map the resistant locus. Among the 600 selected SSR primers, there were four primer pairs exhibiting the polymorphisms between DNA bulk of resistant and susceptible as well as both parents. Segregation distribution of the SSR markers, satt 414 and satt384, were conformed significantly to be as 1:2:1.of Mendel Segregation. The marker, satt411 and satt384, were assigned to link with the resistant locus based on estimation of MAPMAKER/EXP 3.0 software. The linkage order and distance would be Satt384 -5.7cM -Satt411 -7.9cM - Hrcs7 . The resistant locus was integrated to soybean linkage group E based on the Cregen ' s genetic linkage map of soybean.

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