Preliminary Investigations into Use of Metomidate for Euthanasia of Ornamental Fishes

Abstract

The goals of this study were to investigate the potential use of metomidate for one-step euthanasia of ornamental fish species representing commonly sold families in the ornamental fish trade and to determine a baseline euthanasia dose for most species tested. Metomidate hydrochloride, a rapid-acting, water-soluble, nonbarbiturate hypnotic related to etomidate, was tested at various concentrations and durations for euthanasia of species representing the following freshwater and marine aquarium fish families: Apogonidae, Ariidae, Blenniidae, Callichthyidae, Characidae, Cichlidae, Cyprinidae, Gobiidae, Gyrinocheilidae, Loricariidae, Melanotaeniidae, Osphronemidae, Pimelodidae, Poeciliidae, Pomacentridae, and Pseudochromidae. Fish in each trial were euthanized as a group (n=1). Most groups contained 10-12 fish. Some higher doses required buffering. Euthanasia was considered successful if all fish in each group did not recover after 6h in unmedicated water. All species immediately lost buoyancy and equilibrium, dropping to the tank bottom within 1min and ceasing ventilation typically within minutes. However, reactivity to vibration, sound, or touch was noticeable for varying time periods afterward (8-66min), so an additional 30min of exposure after cessation of reactivity was included as part of the protocol. Although some species (Neon Tetras Paracheirodon innesi and Australian Rainbowfish Melanotaenia australis) were euthanized at a concentration of 40mg/L metomidate for a total exposure time of 38min, most species tested were successfully euthanized with metomidate at a concentration of 100mg/L, with total exposure times ranging from 35-96min. A few catfish species (Otocinclus sp. and Bronze Corydoras Corydoras aeneus) were refractory and recovered after 100mg/L. However, Otocinclus sp. were successfully euthanized at 250mg/L, and Bronze Corydoras were euthanized at 1,000mg/L. Further studies are needed to provide additional data for these and other species and families, for different water chemistry conditions, and for various biological factors to fine-tune dosing regimens.