Preliminary Genetic Map of a New Recombinant Inbred Line Population for Narrow-leafed Lupin (Lupinus angustifolius L.)

Bartosz Kozak,Ewa Sawicka-Sienkiewicz,Dariusz Zalewski,Renata Galek

doi:10.3390/agronomy9100653

Bartosz Kozak, Ewa Sawicka-Sienkiewicz + Show 2 more

Open Access

https://doi.org/10.3390/agronomy9100653

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Abstract

Genetic maps are an essential tool for investigating molecular markers’ linkage with traits of agronomic importance. Breeders put a lot of emphasis on this type of markers, which are used in breeding programs implementation and speed up the process of a new variety development. In this paper, we construct a new, high-density linkage genetic map for Polish material on narrow-leafed lupin. The mapping population originated from crossing the Polish variety ‘Emir’ and the Belarusian breeding line ‘LAE-1’. A new map was constructed based on DArTseq markers—a new type of marker generated with the next-generation sequencing (NGS) technique. The map was built with 4602 markers, which are divided into 20 linkage groups, corresponding with the number of gametic chromosomes in narrow-leafed lupin. On the new map there are 1174 unique loci. The total length of all linkage group is 3042 cM. This map was compared to the reference genome of narrow-leafed lupin and the CDS sequence for model legume species: emphMedicago truncatula, emphLotus japonicus and Glycine max. Analysis revealed the presence of the DArTseq marker common for all investigated species. We were able to map 38 new, unplaced scaffolds on the new genetic map of narrow-leafed lupin. The high-density genetic map we received can be used for quantitative trait locus (QTL) mapping, genome-wide association study analysis and assembly of the reference genome for the whole genome sequencing (WGS) method

Highlights

In the process of providing food for the world, high-protein cultivated crops of legume plants (Fabaceae family) play a significant role, and their importance will be even more significant in the future [1]
In recent years we could observe a tendency to replace marker systems such as AFLP, Inter Simple Sequence Repeat (ISSR) and RAPD developed in the early 1990s with techniques using next-generation sequencing (NGS) for map construction [38]
The additional 27 ISSR markers added during the construction process were helpful while filling gaps between DArTseq markers

Summary

Introduction

In the process of providing food for the world, high-protein cultivated crops of legume plants (Fabaceae family) play a significant role, and their importance will be even more significant in the future [1]. The leader in the production of vegetable protein is transgenic soybean (Glycine max L.). In Poland, soybean is a marginal crop due to its lengthy vegetation period, pH requirement close to neutral, and requirement of warm soils maintained in high culture. In Poland majority of soils are light acid ones. The architecture of plants is unfavourable trait due to low position of first set of pods on the main shoot. The main goal of this idea is finding a crop that can partially substitute soybean protein (www.bialkoroslinne.iung.pl)

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