Abstract
The power of high performance liquid chromatography (HPLC) for the separation of complex mixtures of peptides from proteins is becoming increasingly evident (1–4 and references in 3). However, the application of HPLC to the separation of proteins is limited. Monch and Dehnen (5) separated artificial mixtures of proteins (5,400 to 450,000 daltons) and Rubenstein et al. used HPLC to purify interferon (6). In preliminary experiments, we have had some success in the separation of human globin chains by HPLC.
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