Preliminary experiments for the preparation of hyaluronic acid from synovial fluid without rheological changes

Abstract

Hyaluronic acid (HA) isolated from synovial fluid by CsCI density-gradient ultracentrifugation show a decrease in the viscoelasticity. Metrizamide, a substance used recently for non-ionic density-gradient ultracentrifugation, do not change the rheology of synovial fluid and HA. Thus it may be possible to isolate HA without altering rheological properties.Key wordsSynovial fluidviscoelasticityhyaluronic acidisopycnic ultracentrifugationmetrizamide