Abstract
Objective To establish an animal experimental method for investigating the effect of fetal myelomeningocele (MMC) repair with chitosan gelatin membrane,and then observe the bladder protective effect of fetal surgery.Methods Fetal myelomeningocele rats were obtained by feeding pregnant rats with retinoic acid.Open abdominal fetal surgery was performed,and repair of MMC using chitosan gelatin membrane as tissue engineering materials.Fetal rats after repair were harvested bofore dilivery.By observing the success rate of surgery and pathological changes,data were analyzed compare to MMC fetus in groups.Results 44 fetuses underwent repairment,and the use of chitosan gelatin membrane patch in fetal surgery was successful in 10 fetuses.Local tissue changes after the repair of MMC.According to the optical density value in immunochemistry staining,the expression of nerve tubulin-β-Ⅲ in the fetal bladder was significantly higher in operation group as compare with MMC control group,and it had no difference to normal control.Condusion Our preliminary study confirmed that chitosan gelatin membrane as tissue engineering materials in the MMC fetal surgery is feasible,and it showed the protection effect to fetal spine and bladder. Key words: Myelomeningocele ; Fetal repair
Published Version
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