Abstract
A recently developed spectrophotometric technique was used to examine the effect of environmental pollutants on pinocytosis by fish macrophages. Spot, Leiostomus xanthurus, and hogchoker. Trinectes maculatus, were captured from the York River, a relatively non-polluted ricer, and from the Elizabeth River, known to be highly contaminated with polynuclear aromatic hydrocarbons (PAHs). Kidney cell types were separated by density gradient centrifugation and single cell suspensions of macrophages were used in in vitro studies. The pinocytic activity of kidney macrophages from spot and hogchoker was determined by means of a colorimetric assay of ingested neutral red dye. It was found that the pinocytic activity of macrophages from spot exposed to contaminated waters did not differ from control values. However, there was a two-fold increase in pinocytic activity in pollutant-stressed hogchoker. It is proposed that hogchoker could serve as an indicator species to monitor pollution effects in the environment.
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