Abstract
The bioluminescence inhibition bioassay using marine bacteria Vibrio fischeri is widely used as a tool to assess the toxicity of chemical compounds, because of the many benefits comprising cost and time effectiveness, rapidness, sensitivity, and ease of operation. In addition, the test is used for various types of organic and inorganic compounds. Due to the insolubility of organic compounds in water, a suitable solubilizer should be applied. The ecotoxicity of the solvent should be negligible in relation to marine bacteria. On account of superior human activities the synthesis of new chemical substances is inextricably linked to the emergence of chemical compounds that are a potential threat on environment. A Microtox system utilizing the 81.9% Basic Test with 14 dilutions was applied to pre-evaluate the ecotoxicity of β-cyclocitral oxime, citronellal oxime and perillaldehyde oxime. Substances solutions with an initial concentration of 0.036% in DMSO were prepared. The preliminary results show that the studied scent compounds are characterized by quite high toxicity. In order to confirm the received ecotoxicity values, it is necessary to carry out additional surveys using another range of concentrations and biotests on organisms at each trophic level.
Highlights
IntroductionA constantly growing request for synthesis of new fragrance components has been observed [1]
Over the last years, a constantly growing request for synthesis of new fragrance components has been observed [1]
The test relies on disrupting the emission of light in optimal environmental conditions as a result of toxic substances acting on the marine bacteria Vibrio fischeri, which lead to the reduction of light emission
Summary
A constantly growing request for synthesis of new fragrance components has been observed [1]. Fragrances with slight changes in the chemical structure may differ significantly in aroma [4]. Development of synthetic methods for the preparation of natural compounds was possible by unravelling their structures. Modern chemistry manners have allowed both the chemical synthesis of compounds existing in nature as components of essential oils or animal products, as well as the discovery of fragrant molecules that do not exist in nature. Due to the high cost of scents isolated from natural sources, more often aroma compounds are prepared via synthesis [4]. The largest amount of fragrance compounds is present in cosmetics, perfumes and household chemicals [6]
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