Preliminary Crystallographic Study of GlutathioneS-Transferase Fused with the Nuclear Matrix Targeting Signal of the Transcription Factor AML-1/CBF-α2

Abstract

A glutathioneS-transferase fused with the nuclear matrix targeting signal (GST–NMTS) of AML-1/CBF-α2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-α2 protein to the nuclear matrix. The crystal belongs to tetragonal space groupP43212 with unit cell dimensionsa=b= 93.4 Å,c= 57.6 Å. There is one GST-fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 Å and are appropriate for structure determination.