Abstract
The oligosaccharide components of the glycoproteins observed in Vero cells infected by the flavivirus Kunjin were separated by gel filtration following exhaustive Pronase digestion of radioactively labeled glycoproteins. The glycopeptides of gp59, gp56, gp53, and NV2 fell into four classes based on size: (i) 3600 to 4200 daltons, (ii) 2800 to 3100 daltons, (iii) 2300 to 2500 daltons, and (iv) 1500 to 1700 daltons. Glycopeptides of the first class were detected in gp59 and gp56, of the second in gp59, gp56, and gp53, and of the third and fourth classes in gp59, gp53, and NV2. Complex oligosaccharides containing fucose, galactose, mannose, and glucosamine were found in the first two classes and possibly in the third. Oligosaccharides in the smallest class were labeled with [ 3H]mannose and [ 3H]glucosamine only.
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