Abstract
The use of wastewater effluents as source of cellulase for enzymatic conversion to fuel is economically and environmentally beneficial. The enzymatic hydrolysis of cellulose to fermentable sugars is a complex step due to substrate-related and enzyme-related effects and their interactions. This study reports activity of cellulase produced by Streptomyces cinnabarinus and S. globosus isolated from wastewater effluents. Both isolates showed secretion of cellulase in preliminary Congo red dye assay. The cellulase activity was confirmed by filter paper assay. The cellulases secreted by both the isolates showed activity in temperature range 30-50 °C and were active in a wide pH range (4-10). The capability of the isolates to survive and degrade cellulose in extreme pH and temperature suggests their possible utilization in biofuel production using pulp and paper waste.
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