Preliminary Assessment of the Antihypertensive and Antioxidative Activities of the Peptides from “Saba” Banana (Musa balbisiana Colla) Flesh

Eugene Clark L Magpantay,Mia Clare Marie B Clemencia,Mary Ann O Torio,Ana Teresa B Sucgang,Teofila D C Villar

doi:10.26534/kimika.v30i1.31-39

Eugene Clark L Magpantay, Mia Clare Marie B Clemencia + Show 3 more

Open Access

https://doi.org/10.26534/kimika.v30i1.31-39

Copy DOI

Journal: KIMIKA	Publication Date: Jun 6, 2019
Citations: 1	License type: cc-by

Affiliation: University of the Philippines Los Baños

Abstract

The crude proteins were isolated using 0.125 M Tris-HCl with 50 mM NaCl at pH 7.4. The protein content of the crude extract was determined using the Lowry assay and was found to be 4.28 mg/mL. The major band which corresponds to the major protein has an approximate molecular weight of 20 KDa. The isolated crude proteins were subjected to enzymatic digestion using pepsin, trypsin, chymotrypsin, and thermolysin for 3, 4, 12, and 24 hours. The 24-hour digest was found to have the highest percent anti-Angiotensin Converting Enzyme (ACE) activity (36.02%) while the 12-hour digest was found to have the highest anti-oxidative activity (33.14%) using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The 24-hour digest was subjected to Ultra-Performance Liquid Chromatography (UPLC) to determine the peptide fraction responsible for the ACE inhibitory activity. Three peptide fractions (PF1, PF2 and PF3) were chosen and PF2 exhibited the highest percent inhibitory activity (32.21) against ACE. PF2 was subjected to thin layer chromatography (TLC) and the possible identity is EK based on the Rf value traveled by glutamic acid (E) (0.43) and Lysine (K) (0.13). In silico analysis was done to correlate the results with the presence of putative peptides with antioxidative and antihypertensive activities. Results showed that antihypertensive peptides EK, GS, TY, FNE, FP, LKA, PT, PP, FAL and antioxidative peptides IR and VPW were found based on the sequence of the protein in “Saba” banana. The presence of the antihypertensive peptide EK was verified using thin layer chromatography (TLC).

Highlights

There have been numerous studies on alternative ways on how to treat some diseases in a natural, cheap and safe way
Flesh from three ripe “Saba” bananas was used for the isolation of the crude proteins because according to Mohapatra et al (2010) the protein content increases as the fruit matures
Results showed that antihypertensive peptides EK, GS, TY, FNE, FP, LKA, PT, PP, FAL and antioxidative peptides IR and VPW were found based on the sequence of these five selected proteins in “Saba” banana

Summary

Introduction

There have been numerous studies on alternative ways on how to treat some diseases in a natural, cheap and safe way. Of these diseases, hypertension and the diseases caused by oxidative stress are the two being dealt with. Hypertension is usually defined by the presence of a chronic elevation of systemic arterial pressure above a certain threshold value (Giles et al, 2009). According to Chopra and Ram (2019), the latest Europian guidelines regarding hypertension sets the threshold value of blood pressure to be 140/90 mm Hg while the American guidelines lowered the threshold to 130/80 mm Hg. According to the World Health Organization (WHO) data published in 2017, hypertension death rate in the Eugene Clark L.

Objectives

Results

Conclusion