Preliminary and Biochemical Characterization of Bacterial Strains Isolated from Ramie Cane Soils

Abstract

For the present study a total of 18 bacterial isolates were isolated from Ramie canes soils. Preliminary characteristics like colony characteristics and substrate concentrations were studied for the microorganisms. Physicochemical factors affecting the bacterial growth. The three seasons winter, summer and rainy seasons were also affected the growth of the bacteria. Various substrates like starch peptone, xylan peptone, Glycerol, Aspargine, glucose nitrate agar, carboxymethyl cellulose agar and pectin peptone agar media were studied. Maximum colony growth was recorded in pectin peptone agar media. Cream white, orange, red, yellow, lemon yellow and watery white colonies were recorded. The size of the bacterial strains was recorded in 1-55 mm in diameter. All the strains were entire lobed, convex having smooth and rough surface. of the 18 strains 11 were gram positive rods and the remaining strains were negative rods. Among the 18 strains, we were selected six strains for further studies like effect of seasons on growth and amino acid infilteration of bacteria. Maximum growth was recorded in winter season with 85.0 colony count in the presence of CCLS strain. There are 10 amino acids were introduced into the bacteria, with maximum growth was WCES bacterial strain. The results of this study reveal that the soil microflora associated with the ramie cane soils. These bacterial strains were used in the process of decortication and degumming.