Abstract
The study analyzed 48 Old Kladruber horses genotyped by Illumina Equine SNP70 BeadChip for usefulness of genomic data in determining of mating plan. Totally 12 variants of data filtering and their impact on calculations in dependence of different parameters of GenCall Score, Minor Allele Frequency and assumed average values of loci of ancestors (l) was investigated. For possibility of comparison between genomic and commonly evaluated relationships, pedigree based relationship matrix was constructed and subsequently subtraction of pedigree from genomic matrix was performed. All matrices were thoroughly inspected and most suitable setting of parameters was chosen. Evaluation of genomic relationships can be successfully implemented in more precise method of mating plan design of Old Kladruber horses. Further genotyping and development of method for rescaling of differences between genomic and pedigree relationship matrices´ elements is advised for a purpose of better interpretation of results by breeders.
Highlights
New methods of analyses developed in last decade enabled to use more detailed genetic information about animals
Old Kladruber horse as UNESCO heritage of mankind and Czech official genetic resource is aim of research for improvement of breeding conservation program and diversity of population by microsatellites was investigated by Vostrý et al, 2011
Our study is focused on development of method of construction of genomic relationships from SNPs identified by biochips and their practical implementation in mating plan of Old Kladruber horse
Summary
New methods of analyses developed in last decade enabled to use more detailed genetic information about animals. One of these methods utilizes massive detecting of single nucleotide polymorphisms by biochips and the information is used in single-step and multi-step prediction of genomic BLUP breeding values of cattle. Old Kladruber horse as UNESCO heritage of mankind and Czech official genetic resource is aim of research for improvement of breeding conservation program and diversity of population by microsatellites was investigated by Vostrý et al, 2011. Our study is focused on development of method of construction of genomic relationships from SNPs identified by biochips and their practical implementation in mating plan of Old Kladruber horse
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