Abstract
In the past we sometimes found poor cell morphology and relatively low biochemical values for osteoblast cultures on bioactive glass. These observations were not caused by any external causes. Our hypothesis is that the surface reactivity of polished bioactive glass slides might decrease slowly due to the influence of (air-) humidity during storage under normal room conditions. In the present study we investigated the ageing of bioactive glass stored under room conditions as well as bioactive glass stored under dry conditions. We also compared the results with glass slides stored for about one year with freshly obtained bioactive glass slides, both stored under dry conditions. We evaluated several histological and biochemical parameters obtained from osteoblast cultures on the differently preserved glass slides. The results showed that bioactive glass probably maintains its bioactive nature when stored under dry conditions. We found different biochemical values for bioactive glass prepared with a new oven and elaborated with new polishing techniques in comparison with previously prepared batches of bioactive glass. These results indicate that different bioactive glass batches with identical composition may not be identical in terms of bioactivity.
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