Preimplantation Genetic Diagnosis of Embryos Developing From Intracytoplasmic Sperm Injection of In Vitro Matured Metaphase II Human Oocytes

Abstract

The recovery of immature human oocytes is a common occurrence in routine IVF. While in vitro maturation (IVM) of germinal vesicle (GV) or Metaphase I (MI) stage oocytes can be accomplished in conventional culture medium, the viability of these oocytes is questionable. Cell cycle regulatory mechanisms could be altered, and although fertilization can be achieved, the downstream affects of IVM are largely unknown. Here, we assessed chromosomal status of the embryos that developed from in vitro matured MI oocytes which had reached the Metaphase II stage at the time of ICSI which was performed within four hours post egg retrieval.