Preharvest and postharvest UV radiation affected flavonoid metabolism and antioxidant capacity differently in developing blueberries (Vaccinium corymbosum L.)

Abstract

Flavonoids can protect plants against UV but the mechanism by which specific flavonoids during fruit development is unclear, especially in blueberries on living plants. We analyzed the gene expression and metabolite profiles of flavonols, proanthocyanidins (PAs), and anthocyanins under preharvest UV-B/-C and postharvest UV-A/-B/-C irradiation in developing blueberries. Both pre- and postharvest UV irradiation significantly increased flavonol accumulation during early fruit development, while increased anthocyanin and PA contents during late fruit development. However, PAs decreased during postharvest but increased during preharvest UV irradiation in green fruit. The antioxidant capacity increased by postharvest UV irradiation, while hardly affected by preharvest UV irradiation. Overall, the gene expression changes paralleled the flavonoid contents after UV irradiation. Notably, VcMYBPA1 was closely related with VcLAR and VcANR under pre- and postharvest UV irradiation, which could relate to PA biosynthesis. During natural fruit maturation and UV conditions, the elevated PA content exhibited higher potential antioxidant activity. Our results show that UV resistance is greater in living plants than detached fruits, the former showing a systemic and moderate response and the latter a non-systemic but strong response. These results might contribute to the development of pre- and postharvest technologies to promote healthier fruit consumption.