Pregnancy Outcomes of Single/Double Blastocysts and Cleavage Embryo Transfers: a Retrospective Cohort Study of 24,422 Frozen-Thawed Cycles

Xiaoyu Long,Fangrong Wu,Jie Qiao,Lixue Chen,Rong Li,Weiping Qian,Yuanyuan Wang

doi:10.1007/s43032-020-00247-x

Abstract

This study aims to evaluate the effect of blastocyst- and cleavage-stage embryo transfers with different numbers of transferred embryos on pregnancy outcomes in China. This was a retrospective cohort study that collected 24,422 frozen-thawed embryo transfer (FET) cycles in two affiliated hospitals of Peking University Health Science Center between January 2015 and May 2018. They were divided into four groups: the single cleavage-stage embryo transfer group (C-1) (763 cycles), double cleavage-stage embryo transfer group (C-2) (13,004 cycles), single blastocyst-stage embryo transfer group (B-1) (7913 cycles), and double blastocyst-stage embryo transfer group (B-2) (2046 cycles). Of the four groups, the live birth rate was the lowest in the C-1 group (11.8%) while it was the highest in the B-2 group (33.6%). However, the B-2 group was accompanied with higher risks of miscarriages, maternal complications, twin births, preterm births, and low birth weight. Compared with the C-2 group, the B-1 group had a lower live birth rate (23.0 vs 29.0%; aOR, 0.78; 95% CI, 0.72–0.85), but also had a lower risk for twin births (1.9 vs 23.4%; aOR, 0.06; 95% CI, 0.04–0.09) and preterm births (9.6 vs 16.1%; aOR, 0.51; 95% CI, 0.41–0.65). The probability of live birth in the B-1 group declined from 0.25 at 20–29 years old to 0.08 at > 40 years old, while the probabilities of adverse outcomes went up with maternal age. It can be concluded that single-blastocyst embryo transfer seems to be the best choice for all maternal ages. This group of embryo transfer has significantly reduced adverse neonatal outcomes. Especially, women with younger maternal age in this group appear to prominently benefit from single-blastocyst transfer.

Highlights

Xiaoyu Long and Yuanyuan Wang contributed to this work.Electronic supplementary material The online version of this article contains supplementary material, which is available to authorized users.It has been over 40 years since the development of assisted reproductive technology
Maternal characteristics among the four groups were statistically different for maternal age, body mass index (BMI), infertility type, cause of infertility, duration of infertility, type of fertilization in the current cycle, and previous ART cycles (P < 0.05) (Supplementary 1)
It can increase the incidence of miscarriages, fetal deaths, fetal malformations, fetal intrauterine growth restrictions, and the incidence of pregnancy complications such as preterm birth, maternal anemia, pregnancy-induced hypertension, gestational diabetes, and postpartum hemorrhage [11,12,13]

Summary

Introduction

It has been over 40 years since the development of assisted reproductive technology. Single-cleavage embryo transfer and double-blastocyst embryo transfers have been commonly performed in the clinic. Previous studies demonstrated that women who undergo fresh blastocyst transfers achieve higher live-birth rates compared with those who receive fresh cleavage-stage transfers [1]. Other studies have proposed that single-blastocyst embryo transfer could increase the implantation rate but has the same live birth rate as double-cleavage embryo transfer. Another study pointed out that obstetric and perinatal outcomes after blastocyst transfer were similar when compared with embryo cleavage-stage transfers [7, 8]

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Conclusion