Pregnancy-Associated Alterations of Peripheral Blood Immune Cell Numbers in Domestic Sows Are Modified by Social Rank.

Christiane Schalk,Sonja Schmucker,Ulrike Weiler,Birgit Pfaffinger,Volker Stefanski

doi:10.3390/ani9030112

Christiane Schalk, Sonja Schmucker + Show 3 more

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https://doi.org/10.3390/ani9030112

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Abstract

Simple SummaryDuring pregnancy, the maternal immune system is characterized by changes in various immune functions. Nevertheless, pregnancy-associated immune changes and the interplay of rank-associated and gestation-induced immunomodulations are still poorly investigated in sows. Important aspects of blood cellular immunity, cortisol concentrations, and the influence of social rank position during pregnancy were investigated. The results showed that the numbers of various lymphocyte subpopulations decreased, while neutrophils and plasma cortisol concentrations increased during pregnancy. Those pregnancy-associated alterations in the immune system were affected especially in middle-ranking sows, indicating that social rank can influence the immune system and endocrine status in sows during pregnancy.During pregnancy, the maternal immune system is characterized by a shift from adaptive to innate immune functions. Besides, the immune system can be influenced by social rank. Detailed knowledge of pregnancy-associated immune changes and of the interplay of rank-associated and gestation-induced immunomodulations is still fragmentary in sows. This study investigates both the numbers of various blood leukocyte subpopulations during pregnancy and the influence of social rank position on progressing pregnancy-associated alterations in group-housed sows. Sows were classified as low (LR), middle (MR), or high-ranking (HR). Five blood samples were collected from each of the 35 sows throughout pregnancy to evaluate the distribution of blood lymphocyte subpopulations and plasma cortisol concentrations. The numbers of T, natural killer (NK), and B cells, cytotoxic T cells (CTL), and CD8+ γδ- T cells decreased during the last trimester of pregnancy, while neutrophils and plasma cortisol concentration increased before parturition. Social rank revealed different effects on B cells and monocytes with MR sows showing higher numbers than LR sows. Plasma cortisol concentrations also tended to be higher in MR sows as compared to LR sows. In conclusion, sows show pregnancy-associated alterations in the immune system, which are influenced by social rank, as middle-ranking sows in particular display signs of stress-induced immunomodulations.

Highlights

The maternal immune system undergoes unique changes during gestation
Numbers of blood immune cells and cortisol concentrations during pregnancy are shown in Precisely, this pattern was found in T cells, natural killer (NK) cells, B cells, CTL, and CD8+ γδ-T cells
The present study provides a detailed picture of blood immune cell numbers during the entire pregnancy in sows

Summary

Introduction

The maternal immune system undergoes unique changes during gestation. Profound alterations in numbers and functionality of immune cells occur from the first trimester of pregnancy onwards. These adjustments are generally characterized by a shift from adaptive to innate immune functions [1,2]. On the level of the peripheral blood, this change is reflected in increased numbers of circulating. Cells decrease [3,4,5,6,7] This immune status, unique to pregnancy, is crucial for reproductive performance by achieving a successful pregnancy, and for maternal health [8,9,10]. Previous research investigated either broad measures of leukocyte blood counts [11,12,13,14] or an analysis of functionally distinct lymphocyte subsets, such as T helper (TH) cells, cytotoxic T cells (CTL), B cells, and NK cells, was not conducted during the entire pregnancy [11,15,16]

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