Pregnancy achieved in recurrent pregnancy loss patients by art with sperm sorted using Annexin V microbeads

Abstract

ObjectiveTo treat recurrent pregnancy loss couples with ART after annexin V sperm sorting.DesignA total of 62 ART cycles for recurrent pregnancy loss patients were investigated in this study. All couples that involved in this experiment had at least two times of missed abortion. They were divided into three groups according to the sperm preparation method.Materials and MethodsThe sperm and anti-annexin V antibody conjugated microbead are mixed for 15 min, the damaged sperm and microbead bind together. This complex cannot pass through the magnetized column, so we can prepare healthy sperms in the pass-through fraction. We carried out tunnel assay and halo assay to examine DNA damage and apoptosis. Also we performed immuneflorescence staining using Annexin V antibody.ResultsTabled 1The comparison of three groups according to sperm preparation methodsSwim-up group(habitual abortion)Annexin V group(habitual abortion)swim-up (non habitual abortion)number of case3725231ET case3725227age37.3±4.237.8±4.535.6±3.78cycle No3.1±2.73±2.32.18±1.77Retrieval number of oocyte15.9±12.411.6±10.113.7±2.7ET Number3.0±1.53.2±0.93.6±0.64chemical pregnancy43%(16/37)52%(13/25)39.6%(90/227)implantation rate13.7%(15/109)16.2%(13/80)12.4%(113/909)on-going pregnancy rate24.3%(9/37)52%(13/25)28.2%(64/231)missed abortion rate13.5%(5/37)0%(0/25)3.46%(8/231) Open table in a new tab ConclusionIn recurrent pregnancy loss patients, ART with sperm sorted using Annexin V microbeads can be a solution to overcome miscarriage. ObjectiveTo treat recurrent pregnancy loss couples with ART after annexin V sperm sorting. To treat recurrent pregnancy loss couples with ART after annexin V sperm sorting. DesignA total of 62 ART cycles for recurrent pregnancy loss patients were investigated in this study. All couples that involved in this experiment had at least two times of missed abortion. They were divided into three groups according to the sperm preparation method. A total of 62 ART cycles for recurrent pregnancy loss patients were investigated in this study. All couples that involved in this experiment had at least two times of missed abortion. They were divided into three groups according to the sperm preparation method. Materials and MethodsThe sperm and anti-annexin V antibody conjugated microbead are mixed for 15 min, the damaged sperm and microbead bind together. This complex cannot pass through the magnetized column, so we can prepare healthy sperms in the pass-through fraction. We carried out tunnel assay and halo assay to examine DNA damage and apoptosis. Also we performed immuneflorescence staining using Annexin V antibody. The sperm and anti-annexin V antibody conjugated microbead are mixed for 15 min, the damaged sperm and microbead bind together. This complex cannot pass through the magnetized column, so we can prepare healthy sperms in the pass-through fraction. We carried out tunnel assay and halo assay to examine DNA damage and apoptosis. Also we performed immuneflorescence staining using Annexin V antibody. ResultsTabled 1The comparison of three groups according to sperm preparation methodsSwim-up group(habitual abortion)Annexin V group(habitual abortion)swim-up (non habitual abortion)number of case3725231ET case3725227age37.3±4.237.8±4.535.6±3.78cycle No3.1±2.73±2.32.18±1.77Retrieval number of oocyte15.9±12.411.6±10.113.7±2.7ET Number3.0±1.53.2±0.93.6±0.64chemical pregnancy43%(16/37)52%(13/25)39.6%(90/227)implantation rate13.7%(15/109)16.2%(13/80)12.4%(113/909)on-going pregnancy rate24.3%(9/37)52%(13/25)28.2%(64/231)missed abortion rate13.5%(5/37)0%(0/25)3.46%(8/231) Open table in a new tab ConclusionIn recurrent pregnancy loss patients, ART with sperm sorted using Annexin V microbeads can be a solution to overcome miscarriage. In recurrent pregnancy loss patients, ART with sperm sorted using Annexin V microbeads can be a solution to overcome miscarriage.