Prefreezing as a method enabling animals to survive freezing at an extremely low temperature.

Abstract

IN a previous paper it was demonstrated that, after sufficient extracellular freezing, some intact Insects could survive freezing in liquid oxygen without any antifreeze agent1. This might be a method for keeping an entire organism alive at extremely low temperatures, provided that it is sufficiently frost-resistant. Some work along these lines has been carried out in our laboratory. In plant tissues, Sakai has already successfully applied the method to various hard woods2. In animal material it was examined with regard to various intact animals and to excised tissues or cells.