Preferred carbon precursors for lipid labelling in the heterotrophic endosperm of developing oat (Avena sativa L.) grains

Abstract

Oat (Avena sativa L.) is unusual among the cereal grains in storing high amounts of oil in the endosperm; up to 90% of total grain oil. By using oat as a model species for oil metabolism in the cereal endosperm, we can learn how to develop strategies to redirect carbon from starch to achieve high-oil yielding cereal crops. Carbon precursors for lipid synthesis were compared in two genetically close oat cultivars with different endosperm oil content (about 6% and 10% of grain dw, medium-oil; MO, and high-oil; HO cultivar, respectively) by supplying a variety of 14C-labelled substrates to the grain from both up- and downstream parts of glycolysis, either through detached oat panicles in vitro or by direct injection in planta. When supplied by direct injection, 14C from acetate was identified to label the lipid fraction of the grain to the highest extent among substrates tested; 46% of net accumulated 14C, demonstrating its applicability as a marker for lipids in the endosperm. Time course analyses of injected 14C acetate during grain development suggested a more efficient transfer of fatty acids from polar lipids to triacylglycerol in the HO as compared to the MO cultivar, and turnover of triacylglycerol was suggested to not play a major role for the final oil content of oat grain endosperm despite the low amount of protective oleosins in this tissue. Moreover, availability of light was shown to drastically affect grain net carbon accumulation from 14C-sucrose when supplied through detached panicles for the HO cultivar.