Abstract
Peanut agglutinin (PNA), a d-galactose specific lectin, agglutinated 10–18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA + cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA − cells. The specific activity of deoxycytidine kinase as well as the incorporation of [5- 3H]deoxycytidine were also much higher in PNA + cell fraction than in PNA − fraction (7.7-fold and 6-fold, respectively). On the other hand, thymidine kinase activity and [5- 3H]deoxythymidine incorporation were only 3.6 and 3.9 times higher, respectively. The data presented here show a high degree of DNA synthesis and preferential utilisation of [5- 3H]deoxycytidine for DNA synthesis in this undifferentiated B lymphocyte fraction.
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