Abstract
Cellular entry mediators define whether the cell is permissive to PRRSV infection. Porcine sialoadhesin (pSn, Siglec-1) and CD163 are main entry mediators facilitating infection of porcine macrophages by PRRSV. Recently, Siglec-10 was demonstrated to be an alternative receptor for PRRSV. To examine if virulence and pathogenicity of PRRSV strains could be correlated with the use of different Siglecs, a PK15 cell line recombinantly expressing Siglec-1 and CD163 (PK15S1–CD163) and a PK15 cell line recombinantly expressing Siglec-10 and CD163 (PK15S10–CD163) were used to compare the virus replication of 7 genotype 1 subtype 1 strains (G1s1), 2 genotype 1 subtype 3 (G1s3) strains and 5 genotype 2 (G2) strains. Some strains (08VA (G1s1), 13V117 (G1s1), 17V035 (G1s1), VR2332 (G2)) were poor virus producers (<104 TCID50/mL), while other strains (07V063 (G1s1), 13V091 (G1s1), Su1-Bel (G1s3), MN-184 (G2), Korea17 (G2) and SDSU-73 (G2)) easily grew up to ≥106 TCID50/mL. PK15S10–CD163 cells exhibited a higher efficiency in virus production per infected cell than the PK15S1–CD163 cells. The G1s1 strains LV and 07V063 infected more cells in the PK15S1–CD163, whereas the 94V360 and 08VA strains preferred PK15S10–CD163. The highly virulent G1s3 strains Lena and Su1-Bel showed a strong preference for PK15S1–CD163. The G2 strains MN-184, SDSU-73, Korea17 had a much higher infection rate in PK15S10–CD163, while the reference strain VR2332 and the NADC30 strain had a slight preference for PK15S1–CD163. Differences in receptor use may influence the outcome of a PRRSV infection in pigs and explain in part the virulence/pathogenicity of PRRSV strains.
Highlights
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the Arterivirus, genus, family Arteriviridae, order Nidovirales [1] causing respiratory disorders in piglets and reproductive problems in adult animals
The cells maintained a stable expression of sialic acid-binding immunoglobulin-type lectins (Siglec)-10 and CD163 for at least 15 passages. PK15S1–CD163 cells were previously produced by Delrue et al [10]
Analysis of Siglec‐1, Siglec‐10 and CD163 surface and cytoplasm expression in PK15S1–CD163 and PK15S10–CD163 cells Based on the results of the binding and internalization assays, we found that irrespective of the PRRSV strain more virus particles were attached and internalized in the PK15S1–CD163 cells
Summary
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the Arterivirus, genus, family Arteriviridae, order Nidovirales [1] causing respiratory disorders in piglets and reproductive problems in adult animals. The virus infects a subpopulation of tissue macrophages, and subpopulation of monocyte and bone marrow derived dendritic cells [4–9]. Efficient PRRSV replication is observed in primary porcine alveolar macrophages (PAM), differentiated monocytes [10] and for. Xie et al Vet Res (2018) 49:67 facilitate the infection of non-permissive cells by PRRSV [18]. To analyze the receptor use of different PRRSV strains (7 G1s1, 2 G1s3 and 5 G2), a stably transfected cell line expressing both Siglec-10 and CD163 (PK15S10–CD163) was established and compared with the earlier developed cell line stably expressing both Siglec-1 and CD163 (PK15S1–CD163) [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
