Abstract
THE effects of dye solvents on the selectivity of histo-logical staining have not been extensively investigated. Robards and Purvis1 have reported preferential staining of the gelatinous fibres of tension wood by chlorazol black E when dissolved in methyl cellosolve. Earlier the influence of solvents on the staining reactions of this dye was discussed by Cannon2. Puchtler and Sweat3 reported a method for selectively staining haemoglobin in sections with amido black in a methanol solution, but not in water. Investigation of phospholipid staining by Luxol fast blue G revealed a changing selectivity dependent on the solvent used4. This dye in methanol solution was later found to stain only collagen and elastin5.
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