Preferential increase of IL-2R + CD4 + T cells and CD45RB − CD4 + T cells in the central nervous system in experimental allergic encephalomyelitis

Abstract

We examined lymphocytes isolated from the spinal cord (SC), peripheral blood (PB) and lymph nodes (LN) draining the immunization site of Lewis rats with acute experimental allergic encephalomyelitis (EAE). Cells were analysed for T cell subset markers CD4 (mAb W3/25) and CD8 (mAb OX8), for IL-2R (mAb OX39), and for high molecular mass leukocyte common antigen (LCA, CD45RB) expression (mAb OX22). T cells expressing high (CD45RB +) or low (CD45RB −) molecular mass LCA are of different maturational stages and/or separate lineages. CD4 + T cells were more predominant in SC than in PB and LN; CD8 + T cells were scarce in SC but common in PB and LN. Activated CD4 + T cells (IL-2R +) were common in the SC and LN but infrequent in blood. CD4 + T cells that were CD45RB + were scarce in the SC. In contrast, the majority of CD4 + T cells in the PB and LN were CD45RB +. The preferential accumulation of IL-2R + CD4 + T cells and of CD45RB − CD4 + T cells in the central nervous system (CNS) indicates that a selective mechanism directs cell egress into CNS lesions in EAE.a