Pre-evaluation of urease production by Bacillus sp. SK II-5 thermophilic bacteria using agricultural waste as a substrate

Abstract

Agricultural waste contain nutritive minerals including nitrogen, phosphorus, and potassium which help plants grow faster and increase yields, but when released into the open environment can leads the aquatic environment to eutrophication. Agricultural waste, which is mostly contain urea, can be utilized for the growth media of microorganisms to produce urease. Urease is an enzyme that functions to hydrolyze urea to ammonia and carbonate. Naturally, some organisms can produce urease, one of which is the thermophilic bacteria of the genus Bacillus. The purpose of this research is determine the results of the viability test of thermophilic bacteria Bacillus sp. SK II-5 using small island agricultural waste as media. The viability test of Bacillussp. SK II-5 is carried out using the streak plate and turbidimetry methods. The results of this research were thermophilic bacteria Bacillus sp. SK II-5 can grow at 50°C and 60°C which have been tested for cell viability in agricultural waste extract agar media at 37°C.