Predisposition of IL-1β (-511 C/T) polymorphism to renal and hematologic disorders in Indian SLE patients

Abstract

ObjectiveTo see the role of IL-1β gene polymorphisms to disease susceptibility in Indian SLE patients. Materials and methodsThis case-control study included 200 SLE patients and 201 controls. Serum levels of IL-1β and anti-C1q antibodies were measured by multiplex immunoassay and ELISA respectively. Genotyping of SNPs in IL-1β (-511C/T and +3954C/T) were performed by PCR-RFLP method. Autoantibody profile was done in 141 SLE patients. ResultsMean serum levels of IL-1Β were significantly higher (10.9±5.3pg/ml) in patients compared to controls (7.0±2.0pg/ml) (p<0.0001). Similarly, mean levels of IL-1β were significantly elevated among patients with renal and hematologic manifestations. IL-1Β levels were negatively correlated with hemoglobin concentration (r=−0.25, p=0.001). A statistically significant difference was observed for IL-1β -511CC genotypes (OR=2.9, p=0.0068, Pc=0.0136) in patients with Lupus Nephritis (LN) as compared to SLE patients without nephritis. The IL-1β+3954CT genotype (OR=2.0, p=0.0032, Pc=0.0064) and +3954T allele (OR=1.9, p=0.0017, Pc=0.0034) showed significantly higher frequency among patients as compared to controls. Furthermore, the -511C allele showed higher prevalence in patients with renal (OR=1.6, p=0.0335) and hematologic (OR=1.6, p=0.0326) manifestations among SLE patients. ConclusionIL-1β gene polymorphisms appear to confer susceptibility to the disease in Indian SLE patients.