Predictive Value of NKILA and lnc13 lnc RNAs for Distinguishing of Celiac Disease and IBD: A Case-Control Study

Abstract

Long-noncoding RNA 13 (lnc13) and NF-κB-interacting lncRNA (NKILA) are long noncoding RNAs (lncRNAs) that play a role in inflammatory disorders pathogenesis. This study aimed at investigating the expression of these lncRNAs in inflammatory bowel disease (IBD) and celiac disease (CeD) patients compared to controls. We evaluated mRNA expression of lnc13 and NKILA in peripheral blood and intestinal biopsy samples of 50 CeD, 46 IBD patients, and 20 controls using qRT- PCR method. Compared to the controls, CeD and IBD patients had a significantly higher PBMC mRNA level of Lnc13 (p < 0.0001 for both of them) and NKILA (p < 0.0001 and p=0.0174, respectively). NKILA mRNA level was significantly higher in CeD than in IBD patients (p < 0.0001). IBD subjects had significantly increased colonic NKILA expression (p=0.004). In UC, NKILA, and CD, lnc13 colonic expression was significantly increased compared to others (p=0.002 and p=0.0111 respectively for UC, p = 0.0075 and p = 0.002, respectively for CD). Collectively, increased peripheral expression of lnc13 and NKILA along with their decreased duodenal expression demonstrates CeD. Moreover, suppose the increased peripheral expression of lnc13 and NKILA is associated with increased lnc13 colonic expression. In that case, it indicates CD, and if it is associated with increased NKILA colonic expression, it indicates UC.