Predictive role of indicators of oxidative metabolism and elemental status in professional drivers of vehicles in the Northern region

Abstract

Introduction. The predominant source of environmental pollution is motor transport, the release of pollutants which leads to excessive formation of free radicals. The most important metabolic part of the non-specifi c component of the syndrome of adaptation to adverse factors is the activation of free radical oxidation processes that contribute to the mobilization of protective reserves of the body. There is evidence that different groups of the population have different vulnerability to Prooxidant exposure to pollutants. The aim of the study is to establish correlations between the indicators of the pro — and antioxidant system and the elemental status of professional drivers living in the Khanty-Mansiysk Autonomous Region. Materials and methods. 182 male residents (32.6±6.2 years old) of the Khanty-Mansiysk Autonomous Region (KHMAR) were examined: 94 drivers of heavy trucks and fuel trucks and 88 employees. Free radical oxidation products (FRO) were determined in the blood: lipid hydroperoxides (LHP) and thiobarbituric acid active products (TBA-AP) using test kits from BCM Diagnostics (Germany) and AGAT (Russia). Total antioxidant activity (TAA) and thiol status (TS) were determined using commercial kits from Cayman Chemical and Immundiagnostik AG (Germany). The coefficient of oxidative stress (COS) was calculated using the formula: COS=LHP × TBA-AP / TAA × TS. The content of vitamin E was determined on the fl uorometer “Fluorat 02 — ABLF” of the company “Lumex” (Russia), vitamin C — using commercial kits of the company “Immundiagnostik AG” (Germany) on the analyzer of the company “Personal Lab” (Italy). The concentration of macro — and microelements (Ca, Se, Zn, Fe, Pb, Cd) was determined in the samples of AES — ISP and MS — ISP hair in TcMB (Moscow). Statistical processing was performed using the Microsoft Exsel software package and “Statistica 8.0”. For normal distribution, M, m, min, and max values were used. For abnormal distribution, both Me, 25 and 75 PS were used. Correlation relationships were studied using Spearman’s correlation coefficient. Differences at p<0.05 were considered reliable. Results. Drivers compared with employees have signifi cantly higher values of lipid peroxidation, Pb, Cd, Fe ( p <0.001–0.046), against the background of lower indicators of AOS, Ca, Se, Cu, Zn ( p <0.001–0.040). Direct relationships between TAA, vitamins E, C, Se and Zn (r=+0.312–0.802) and inverse relationships between TAA and Pb, Cd (r= –0.623–0.558) were found in drivers of KHMAR with more than 5 years of work experience, which is associated with the development of an oxidative imbalance, which is characterized by an increase in lipid peroxidation products. Conclusions. The relationship between the indicators of the state of oxidative metabolism, the availability of bioelements and antioxidant vitamins involved in the regulation of metabolic processes (p=0.048–0.001) has been established. Direct relationships between the indicators of total antioxidant activity, the glutathione system and the concentration of vitamins E, C, selenium and zinc (r=+0.312–0.802) and inverse relationships between the level of total antioxidant activity, thiol status, and toxic elements: lead and cadmium (r= –0.513–0.604) in the male population of KHMAR were revealed. More pronounced changes in adaptive metabolic reactions in the body of employees of motor transport companies who have a work experience of more than 5 years are associated with the development of an oxidative imbalance, which is characterized by an increase in POL products. The obtained data on the state of the oxidative status of the body of residents exposed to the negative eff ects of a chemical factor can be the basis not only for the formation of risk groups, the development and implementation of a set of scientifi cally-based preventive measures, but also for subsequent evaluation of the eff ectiveness of their use.