Prediction of theRMvalues of selected methyl esters of higher fatty acids in RPTLC

Abstract

Methyl laurate, methyl myristate, methyl palmitate, methyl isostearate, methyl stearate, and methyl arachidate have been separated by RPTLC on kieselguhr F 254 impregnated with different amounts of paraffin oil, using two different mobile phases. The R M values of these compounds for all the stationary phases and mobile phases investigated could be correlated with the dipole moments of the mobile phases, with the percentage impregnation of the stationary phase, and with numerical values of one topological index from among those based on the distance matrix ( W , A , ° B , 1 B ) or on the adjacency matrix ( 1 X , 1 X v ). The theoretical partition coefficient (log P ) for methyl isostearate was calculated from R M values or from one of the topological indexes mentioned above.