Prediction of the Remaining Activity of Horse Liver Alcohol Dehydrogenase after Exposure to Various Organic Solvents

Abstract

Twenty solvents were selected from a principal component (PC) analysis of 103 organic solvents. The solvents were selected so as to obtain a set of test items that spanned a large range of molecular properties of the selected solvents. The activity of free and Celite-immobilized horse liver alcohol dehydrogenase (HLADH) obtained after 20 h exposure to these solvents were used to create a PLS model based on 14 solvent descriptors. A correlation coefficient of 0.997 between the predicted and the observed remaining activity was obtained.A slightly worse PLS model was obtained using only three solvent descriptors (log P, dielectric constant and melting point). A correlation coefficient of 0.957 between the predicted and the observed remaining activity was obtained. The latter model was used to predict the remaining activity of HLADH after its exposure to 93 organic solvents.