Prediction of Protection against Asian Enterovirus 71 Outbreak Strains by Cross-neutralizing Capacity of Serum from Dutch Donors, The Netherlands.

Sabine M.G Van Der Sanden,Sylvie M Koekkoek,Hetty Van Eijk,Katja C Wolthers,Gerrit Koen,Menno D De Jong

doi:10.3201/eid2209.151579

Abstract

Outbreaks of human enterovirus 71 (EV-71) in Asia are related to high illness and death rates among children. To gain insight into the potential threat for the population of Europe, we determined the neutralizing activity in intravenous immunoglobulin (IVIg) batches and individual serum samples from donors in the Netherlands against EV-71 strains isolated in Europe and in Asia. All IVIg batches and 41%, 79%, and 65% of serum samples from children ≤5 years of age, women of childbearing age, and HIV-positive men, respectively, showed high neutralizing activity against a Dutch C1 strain, confirming widespread circulation of EV-71 in the Netherlands. Asian B3-4 and C4 strains were efficiently cross-neutralized, predicting possible protection against extensive circulation and associated outbreaks of those types in Europe. However, C2 and C5 strains that had few mutations in the capsid region consistently escaped neutralization, emphasizing the importance of monitoring antigenic diversity among circulating EV-71 strains.

Highlights

Enterovirus 71 (EV-71) is a member of the genus Enterovirus, family Picornaviridae, and is a major causative agent of hand, foot and mouth disease in children
To gain more insight into the potential threat of Asian enterovirus 71 (EV-71) outbreak strains for the European population and the potential treatment efficacy of Intravenous administration of human immunoglobulin (IVIg), we determined the cross-neutralizing capacity of IVIg batches composed of plasma from the general population of the Netherlands during 2005–2014 against EV-71 subtypes circulating in Europe or Asia and compared results to IVIg batches from
EV-71 Cross-neutralizing Capacity of IVIg Batches As human immunoglobulin batches represent the immunological profile of the general population, we studied the crossneutralizing capacity of IVIg batches composed of plasma from Dutch, Japanese, and Vietnamese donors against EV71 strains isolated in Europe and Asia (Figure 1)

Summary

Introduction

Enterovirus 71 (EV-71) is a member of the genus Enterovirus, family Picornaviridae, and is a major causative agent of hand, foot and mouth disease in children

Methods

Results

Conclusion