Prediction of prokaryotic and eukaryotic promoters based on information-theoretic features

Abstract

Promoters are DNA regulatory elements located near the transcription start site and are responsible for regulating the transcription of genes. DNA fragments arranged in a certain order form specific functional regions with different information contents. Information theory is the science that studies the extraction, measurement and transmission of information. The genetic information contained in DNA follows the general laws of information storage. Therefore, method in information theory can be used for the analysis of promoters carrying genetic information.In this study, we introduced the concept of information theory to the study of promoter prediction. We used 107 features extracted based on information theory methods and a backpropagation neural network to build a classifier. Then, the trained classifier was applied to predict the promoters of 6 organisms. The average AUCs of the 6 organisms obtained by using hold-out validation and ten-fold cross-validation were 0.885 and 0.886, respectively. The results verified the effectiveness of information-theoretic features in promoter prediction. Considering the possible redundancy in the feature set, we performed feature selection and obtained key feature subsets related to promoter characteristics. The results indicate the potential utility of information-theoretic features in promoter prediction.