Abstract
Peptide separation by normal-phase liquid chromatography was studied in my two previous papers. The present study shows a linear relationship between the logarithm of the capacity factor and the logarithm of the volume fraction of modifier in the mobile phase. This permitted the use of a model to predict isocratic and gradient retention times following two initial gradient runs. An algorithm for predicting was based on a modification of the ion-exchange model reported in the previous paper. Observed retention times were in reasonably good agreement with those predicted.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
