Abstract
BackgroundNarrow-leafed lupin is an emerging crop of significance in agriculture, livestock feed and human health food. However, its susceptibility to various diseases is a major obstacle towards increased adoption. Sclerotinia sclerotiorum and Botrytis cinerea – both necrotrophs with broad host-ranges - are reported among the top 10 lupin pathogens. Whole-genome sequencing and comparative genomics are useful tools to discover genes responsible for interactions between pathogens and their hosts.ResultsGenomes were assembled for one isolate of B. cinerea and two isolates of S. sclerotiorum, which were isolated from either narrow-leafed or pearl lupin species. Comparative genomics analysis between lupin-derived isolates and others isolated from alternate hosts was used to predict between 94 to 98 effector gene candidates from among their respective non-conserved gene contents.ConclusionsDetection of minor differences between relatively recently-diverged isolates, originating from distinct regions and with hosts, may highlight novel or recent gene mutations and losses resulting from host adaptation in broad host-range fungal pathogens.
Highlights
Narrow-leafed lupin is an emerging crop of significance in agriculture, livestock feed and human health food
We found one gene in Bcin-Lang that was missing from reference isolate which was associated with the DnaJ domain (PF00226)
We report the genome assemblies of two isolates of S. sclerotiorum (Sscl-Lang and Sscl-Lmut) and one isolate of B. cinerea (Bcin-Lang), isolated from Western Australian lupin hosts
Summary
Narrow-leafed lupin is an emerging crop of significance in agriculture, livestock feed and human health food. Sclerotinia sclerotiorum and Botrytis cinerea – both necrotrophs with broad host-ranges - are reported among the top 10 lupin pathogens. Narrow-leafed lupin (Lupinus angustifolius L.) is an important grain legume crop of south and west Australia and Northern Europe which significantly contributes to animal feed, aquaculture and sustainable farming because of its nitrogen fixation and use in rotation systems [1]. Pearl lupin (L. mutabilis Sweet., Andean Lupin, tarwi) is a minor indigenous crop in South America for which limited. Both S. sclerotiorum and B. cinerea are necrotrophic Ascomycetes with broad host-ranges [6].
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