Prediction of miRNAs targeting cytokine induced apoptosis inhibitor 1 and their expression in multidrug-resistant breast cancer cell lines

Abstract

Objective To predict and select the targeting miRNA highly matched the 3'untranslated region(3'UTR) of cytokine induced apoptosis inhibitor1 (CIAPIN1) gene, and to primarily investigate the targeting correlation between CIAPIN1 and the target miRNA in parental cell lines and drug-resistant cell lines of breast cancer. Methods The miRNA potentially binding to seed side of CIAPIN1 3'-UTR was predicted by the bioinformatics software as well as screening out the target miRNA according to the literatures TargetScan, PicTarmi and miRanda. The expression of CIAPIN1 protein in parental cell lines and resistant cell lines of breast cancer was detected by using Western blot. The expression of the target miRNA in parental cell lines and resistant cell lines of breast cancer was detected by real-time quantitative PCR (qRT-PCR). Results The miRNAs binding to seed side of targeting CIAPIN1 3'-UTR were predicted including miRNA143, miRNA571, miRNA647. Compared with parental cell lines, the CIAPIN1 protein in resistant cell lines of breast cancer was high expression detected by Western blot with significant difference (MCF-7/Tax vs. MCF-7: 0.80 vs. 0.21; MDA-MB-231/Tax vs. MDA-MB-231: 0.70 vs. 0.31; MDA-MB-453/Tax vs. MDA-MB-453: 1.00 vs. 0.35, all P<0.035). Compared with parental cell lines, miRNA143, miRNA571 and miRNA647 in resistant cell lines was low expression detected by qRT-PCR, and there were statistical differences. Conclusions The miRNAs binding to seed side of CIAPIN1 3'-UTR are predicted and selected including miRNA143, miRNA571, miRNA647. Compared with in parental cell lines, these miRNAs are low expression, and the CIAPIN1 protein is high expression in resistant cell lines of breast cancer, which suggests these miRNAs may be involved in multidrug resistance in breast cancer by regulating CIAPIN1 gene. Key words: Breast neoplasms; Multiple drug resistance; microRNA; Cytokine induced apoptosis inhibitor 1