Abstract
Papillomaviruses are the causing agents of benign tumors in their hosts, i.e., mammals and birds, across the world. They have circular double stranded DNA genome. In order to combat the viral infection in Macaca fascicularis PV type 2, a computational pipeline was employed in this study for the prediction of viral protein targeting peptides for vaccine discovery. Epitope prediction enabled the identification of multi-peptides suitable for vaccine development. Further in-depth analysis for immunogenicity and toxicity prediction scrutinized the optimal candidate for target based designing of vaccines. Immunogenic and physicochemical properties of proteins E1, E2, E4, E6, E7, L1, and L2 of Macaca fascicularis PV type 2 revealed their instability index, molecular weight, and antigenic potential. The predicted epitopes may lead to promising targets for broad spectrum vaccine designing against the viral strain of Macaca fascicularis PV type 2.
Highlights
Papillomaviruses are double stranded DNA viruses identified in healthy skin and melanoma skin from immunocompetent patients (Forslund et al, 2007)
Macaca fascicularis PV type 2 (MfPV2) belongs to betapapilloma group of the family, etiological agent of both benign, and malignant skin lesions mainly in primates similar to that is seen in human population (Chen et al, 2009)
Population Coverage Analysis: Afterwards, population coverage analysis was done by using Immune Epitope Database and Analysis Resource (IEDB) Population Coverage tool available at http://tools.immuneepitope. org/tools/population/iedb_input for the identification of all the possible binding alleles (MHC Class I and MHC Class II)
Summary
Papillomaviruses are double stranded DNA viruses identified in healthy skin and melanoma skin from immunocompetent patients (Forslund et al, 2007) They are associated with mucosal, oral (Parkin and Bray, 2006), epithelial, and cervical cancers (zur Hausen, 2002). Macaca fascicularis PV type 2 (MfPV2) belongs to betapapilloma group of the family, etiological agent of both benign, and malignant skin lesions mainly in primates similar to that is seen in human population (Chen et al, 2009). These include rhesus (Macaca mulatta) (Chan et al, 1997), cynomolgus macaques (Macaca fascicularis) (Antonsson and Hansson, 2002), and pygmy chimpanzees (Pan paniscus) (Van Ranst et al, 1991). The consequential epitopes of the present study would be a germane initiator for potential vaccine development against Macaca fascicularis PV type 2
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