Prediction of DNA-Binding Residues in Local Segments of Protein Sequences with Fuzzy Cognitive Maps.

Abstract

While protein-DNA interactions are crucial for a wide range of cellular functions, only a small fraction of these interactions was annotated to date. One solution to close this annotation gap is to employ computational methods that accurately predict protein-DNA interactions from widely available protein sequences. We present and empirically test first-of-its-kind predictor of DNA-binding residues in local segments of protein sequences that relies on the Fuzzy Cognitive Map (FCM) model. The FCM model uses information about putative solvent accessibility, evolutionary conservation, and relative propensities of amino acid to interact with DNA to generate putative DNA-binding residues. Empirical tests on a benchmark dataset reveal that the FCM model secures AUC = 0.72 and outperforms recently released hybridNAP predictor and several popular machine learning methods including Support Vector Machines, Naïve Bayes, and k-Nearest Neighbor. The improvements in the predictive performance result from an intrinsic feature of FCMs that incorporate relations between the input features, besides the relations between the inputs and output that are modelled by other algorithms. We also empirically demonstrate that use of a short sliding window results in further improvements in the predictive quality. The funDNApred webserver that implements the FCM predictor is available at http://biomine.cs.vcu.edu/servers/funDNApred/.