Abstract
In order to predict B-cell linear epitopes of egg allergen ovotransferrin(OVT),the antigen index,hydrophilicity,and surface accessibility and flexibility of egg OVT were predicted using Jameson-Wolf,Kyte-Doolittle,Emini and Karplus-Schulz methods,respectively.The secondary structure of OVT was predicted by Chou-Fasman and Deleage-Roux methods based on the amino acid sequence of egg OVT derived from Genbank.Based on the above comprehensive analysis,7 possible B-cell linear epitope candidates in egg OVT were predicted to locate in the regions of 174-181,215-222,231-240,288-294,332-344,415-429 and 547-555.These results are useful for fine mapping of B-cell epitopes in egg OVT.
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