Abstract
In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial microspectrometer with near infrared laser, we measured the Raman spectra of gelatin hydrogels at various concentrations. By building a calibration curve from measured refractometry data and Raman scattering intensity for different vibrational modes of the hydrogel, we were able to predict the refractive indices of the gels from their Raman spectra. This work highlights the importance of a correlative approach through Brillouin–Raman microspectroscopy for the mechano–chemical analysis of biologically relevant samples.
Highlights
Gelatin hydrogels derived from denatured collagen[1] constitute a simple model to investigate the physical properties of connective tissue
We have demonstrated that Raman spectroscopy can be applied to assess the refractive index of biologically relevant samples with appropriate calibration
We have shown that this method can be utilized simultaneously with Brillouin spectroscopy, to assess the localized refractive index from the same spatial location as the Brillouin measurement
Summary
Gelatin hydrogels derived from denatured collagen[1] constitute a simple model to investigate the physical properties of connective tissue. Raman microspectroscopy was successfully applied to gelatin hydrogels, used as biological tissue models, to monitor the refractive index of the gels using vibrational bands.
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