Abstract
Understanding protein-protein interactions (PPIs) is vital to reveal the function mechanisms in cells. Thus, predicting and identifying PPIs is one of the fundamental problems in system biology. Various high-throughput experimental and computation methods have been developed to predict PPIs. Here, we provide a straightforward guide of using the program "SPRINT" to predict the PPIs on an interactome level in an organism. First, some installation guides and input file formats are described. Then, the commands and options to run SPRINT are discussed with examples. In addition, some notes on possible extended installation and usage of SPRINT are given.
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