Predicting Protein Mutant Stability With A Combined Experimental/theoretical Approach

Abstract

A complete understanding of the relationships between protein structure, stability and dynamics remains an open biophysics problem. Much of our insight comes from laborious experimental analyses that perturb structure via directed mutation. The glycolytic enzyme lysozyme is among the most well characterized proteins under this paradigm, due to its abundance and ease of manipulation. To speed up such analysis, efficient computational models that can accurately predict mutation effects are needed.