Abstract
Ribotyping is the most widely used method for differentiating strains of Clostridium difficile for epidemiological studies and infection control. Recently there have been calls for standardisation of the technique to which sophisticated technical solutions have been offered. This note offers a solution for standardisation based on conserved rrn operon Type-specific flanking genes. Furthermore, this technique can be used to detect Type-specific rrn operon deletions in passages from a single strain of C. difficile
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