Predicting extinction and reinstatement of alcohol and sucrose self-administration in outbred rats.

Abstract

Preventing relapse to drinking or escalation to excessive drinking could be aided by identifying factors that predict these behaviors. Animal models, particularly those that utilize operant self-administration techniques, can be useful. In a prior operant study, we noted a good deal of variability in behaviors during training and test sessions. We utilized data obtained from that study of two groups of rats, trained and tested identically except one responded for alcohol and the other for sucrose, to explore for associations related to relapse (reinstatement) or to excessive drinking (maintenance). Data were obtained from sessions conducted under fixed- and progressive-ratio schedules as well as from extinction and reinstatement sessions. Variables assessed included active and inactive presses, head entries into the dipper trough, and automated recordings of body movements during these sessions as well as alcohol preference before training. First, using multiple regression, we examined whether alcohol preference before training associated with any response variable among alcohol-responding rats. Second, using factor analysis, we identified a training variable, body movements, that associated with responses during tests. Using this measure, rats were divided into low- and high-response groups and compared on active lever presses and head entries across test sessions. Results show that among alcohol-responding rats, alcohol preference predicted head entries during extinction. High-body-movement rats emitted significantly fewer active lever presses and had fewer head entries across test sessions, particularly during reinstatement, compared with low-body-movement rats. Results from this exploratory study provide clues for future experimental studies.