Abstract
After artificial infection with various isolates of Fusarium graminearum and F. culmorum, kernel infection, deoxynivalenol (DON) and ergosterol contents were assessed by means of visual inspection, RP-HPLC and GC/MS, respectively, in four winter wheat varieties of different Fusarium-resistance. It was found that both DON and ergosterol contents correlated with kernel infection strongly and positively (r=0.8981 and r=0.8945). Similarly, there was a strong, positive correlation (r=0.8737) between DON and ergosterol contents in the infected grains. Since the ergosterol content could be determined with a significantly greater sensitivity than that of the DON, assessment of the former compound can be used as a good and more predictive indicator of Fusarium disease level in winter wheat, requiring minimum amount of grain material. Furthermore, knowledge of the ergosterol concentration in the scabby grains can facilitate the practical application of the quality grading system of Schnurer (1995) to wheat infected by Fusarium spp.
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