Abstract
Bacteriophage is a type of virus that could infect the host bacteria. They have been applied in the treatment of pathogenic bacterial infection. Phage enzymes and hydrolases play the most important role in the destruction of bacterial cells. Correctly identifying the hydrolases coded by phage is not only beneficial to their function study, but also conducive to antibacteria drug discovery. Thus, this work aims to recognize the enzymes and hydrolases in phage. A combination of different features was used to represent samples of phage and hydrolase. A feature selection technique called analysis of variance was developed to optimize features. The classification was performed by using support vector machine (SVM). The prediction process includes two steps. The first step is to identify phage enzymes. The second step is to determine whether a phage enzyme is hydrolase or not. The jackknife cross-validated results showed that our method could produce overall accuracies of 85.1 and 94.3%, respectively, for the two predictions, demonstrating that the proposed method is promising.
Highlights
Bacteriophage, as safe agent, can lyse and infect specific bacteria without destroying natural beneficial microflora (Parmar et al, 2018)
Hydrolytic enzymes encoded by phages are key ingredients of lysis, which is helpful to fighting bacterial pathogens, especially those that cannot be killed by antibiotics and chemicals
The predictive performances of three combined vectors were investigated by using support vector machine (SVM) with jackknife test
Summary
Bacteriophage, as safe agent, can lyse and infect specific bacteria without destroying natural beneficial microflora (Parmar et al, 2018). In some countries, they have been used therapeutically to treat bacterial infections that do not respond to antibiotics (Thiel, 2004; Parfitt, 2005; Keen, 2012). They have been used as a food safety tool to reduce bacterial contamination (Pirisi, 2000). The identification of hydrolases encoded by phages has become an important research topic. It has been studied in chemistry and physics through
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