Abstract
1. 1. The relationship between immunological distance (I.D.) measured by microcomplement fixation and amino acid sequence difference for lysine-rich histones was tested using antisera to lysine-rich histones of known sequence, chicken H1 and H5, goose H5, and trout H1 as well as to trout H5. 2. 2. The best relationship between I.D. ( y) and percent sequence difference ( x) for lysine-rich histones, y = 2 x, applies as well to other histones of known sequence but it differs from y = 5 x, reported for other proteins and often used for histones. 3. 3. Although deviations indicate that I.D. is a poor predictor of primary sequence differences among histones, it suggests that trout H5 is more closely related to H1 than to chicken H5.
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