Preconcentration of imidacloprid with β - cyclodextrin polymer and analysis by HPLC

Abstract

Cyclodextrins are type of oligosaccharide consisting of glucose units. Cyclodextrins are of mainly of three types α, β and γ consisting of six, seven and eight glucose units respectively joined with α-1,4-glycosodic linkage. β-cyclodextrin is a very stable, most useful and low priced oligosaccharide. Guest molecules can be incorporated in its cavity to form supramolecular complex. β-cyclodextrin is modified with cross-linker 1,4-butanedioldiglycidyl ether to form polymer. This sorbent was used as a support for the solid phase extraction of imidacloprid from water samples and these samples were analysed with the help of HPLC. For quantitatative extraction(≥95%) of imidacloprid with β - cyclodextrin polymer various parameters like pH, amount of sorbent, sample volume, contact time and volume of eluent were optimized. The HPLC analysis of imidacloprid was done at λ max at 278nm using 80:20 acetonitrile: water as a mobile phase with a flow rate 1.0ml/min. R t for imidacloprid comes at 2.9 min. Enrichment factor is 75. LOD is 0.45 ng/ml and LOQ is 1.5 ng/ml. Relative standard deviation is 2.1%. The developed method was applied successfully for the determination of Imidacloprid in various water samples.